EKSTRAKSI ENZIM PAPAIN GETAH BUAH PEPAYA UNTUK PROSES DEPROTEINASI PADA PEMBUATAN KITIN DARI KULIT UDANG WINDU DAN APLIKASINYA SEBAGAI BAHAN PENJERNIH SARI BUAH SIRSAK

LILIANTI PUSPANINGRUM

Abstract


Papaya can produce enzyme that often called the enzyme papain. Usually, Enzyme is found in the resin or fruit that is still young. Papaya resin (papain) contains quite a variety of enzyme that is proteolotik (The protein). This enzyme functions as a protein remover (deproteinase) on the skin of the tiger shrimp. Enzyme will be catalyst ligamen of peptyda or protein to simple compound such as amino acids. Currently chitin and chitosan widely used in the processing industry the production process, ie as cleaner on theju ice. Goal of this research is 1) to determine the influence of extraction enzyme papain from papaya fruit resin in the deproteinase process, 2) to determine the influence of the enzyme papain of papaya fruit resin in chitin extraction on making chitin-chitosan and 3) To know the influence of the addition chitosan as cleaner of the soursoap juice. This research was conducted in Laboraturium Technology of Agriculture Faculty of Agriculture, University of Muhammadiyah Malang in April 2009 until complete. The method used in this research is to use the Complete Random Design (CRL), which consists of 2 phases. First Phase, the addition of papain enzyme treatment with 4-level (P0: without papain enzyme, P1: 1% papain enzyme, P2: papain enzyme 3% and P3: 5% papain enzyme) and second Phasetreatment in addition chitosan on soursoap juice with 3 levels (K0: without Chitosan, K1: chitosan control and K2: chitosan papain 5%), each do 3 times a retrial. First Phase combination treatment is the best Chitosan papain treatment 5% produce rendemen 9.22%, 10.48% of water content, protein content 0.67%; chitosan gray level that is 1.19%. For chitosan as cleaner in the soursoap juice show that the addition of chitosan can use as cleaner, marked with pH test, colour intensity, that indicates the result is very significantly with the juice that did not experience additional of chitosan

Keywords


Enzim Papain; Kitosan dan Penjernih; umm