PENGARUH PENGGUNAAN JENIS DAN VOLUME EKSTRAK PUCUK DAUN TANAMAN TERHADAP PERTUMBUHAN MIKROSTEK ANGGREK GRAMMATOPHYLLUM SCRIPTUM L. BLUME.

Khairdin Prmana Jaya

Abstract


This research is a study to test the potency of using tip of plant leaf that is combined with many volume level to know its influence to the orchid (Grammatophyllum scriptum L. Blume) growth with in-vitro techniques (micropropagation).
The hypothesis of this study is : first : there is interaction between volume of extract using and the use extracts of leaf tip to the growth of Grammatophyllum scriptum L. Blume orchid with micropropagation techniques, second : giving extract of leaf plant can fasten the growth of Grammatophyllum scriptum L. Blume orchid with micropropagation techniques, three : increasing higher volume adding can fasten the growth of Grammatophyllum scriptum L. Blume orchid with micropropagation techniques.
This experiment is done on March until June 2002 at Kultur Jaringan Laboratory, Agriculture Biotechnology Center in Muhammadiyah University of Malang. The experiment consists two factors, those are kind of extracts of leaf tip (E) and a number of volume level extracts of leaf tip (K) added one control. It is arranged by using random planning group factorially through Contrast Orthogonal analysis because there is one control. First factor is kind of extracts of leaf tip (E) consists of extract of kumis kucing leaf tip (Orthosiphon aristatos), kembang sepatu (Hibiscus rosa sinensis), mimba (Azadirachta indica), paitan (Eupatorium inulifolium), pecut kuda (Stachytarpheta jaminicensis L. Vahl), and the composition of those extracts of leaf tip. Whereas, the second factor is the volume of extract using (K) that consists of 0 ml extract volume in 1000 ml VW media, 15 ml extract volume in 1000 ml VW media and 30 ml extract volume in 1000 ml VW media. The combination is repeated three times and each treatment consists of 5 bottles of Kultur and added with one control.
The observation is done after plantlet attain the age of 1 week after initiation, with 7 days interval observation time. The observation in growth changing include; leaf number, root number, and shoot number.
The result of observation shows that there is interaction between treatment in using extract of tip leafs and some volume level at shoot number changing on sixth week after initiation with best combination E4K2 (extract of paitan tip leafs (Eupatorium inulifolium) at 15 ml extract volume / 1000 ml VW media). The treatment of using E4 (extract of paitan (Eupatorium inulifolium)) and E5 (extract of pecut kuda (Stachytarpheta jaminicensis L. Vahl)) separately result more average of leaf number and root number than other extracts. Beside that, the higher adding volume of extract of leaf tip (until 30 ml/ 1000 ml VW media) can results more root number. From all control treatments (pure VW media) show better average as effective and efficient plant media to orchid (Grammatophyllum scriptum L. Blume) growth.
Key Word : Extract tip plant leaf, microstek and Grammatophyllum scriptum L. Blume

Keyword : Ekstrak pucuk daun tanaman; mikrostek dan Grammatophyllum scriptum L. Blume

Link terkait : http://skripsi.umm.ac.id/files/disk1/64/jiptummpp-gdl-s1-2005-khairdinpr-3152-Pendahul-n.pdf


Keywords


Ekstrak pucuk daun tanaman; mikrostek dan Grammatophyllum scriptum L. Blume